The Ii41 isoform of invariant chain mediates both positive and negative selection events in T-cell receptor transgenic mice

Immunology. 1998 Nov;95(3):309-13. doi: 10.1046/j.1365-2567.1998.00595.x.

Abstract

The functional role of invariant chain in T-cell selection events and antigen presentation is well established. The invariant chain gene encodes differentially spliced isoforms, Ii31 and Ii41. The Ii41 isoform has been described to increase the efficiency of antigen presentation. We have analysed the effect of the Ii41 isoform on positive and negative selection of transgenic CD4 T cells with specificity for a natural self antigen (C5) which are crucially dependent on invariant chain for their development and functional antigen recognition. The data show that Ii41 fully substitutes for wild-type invariant chain in both positive and negative selection events during functional maturation of T cells with specificity for a natural, blood-borne self antigen.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigen-Presenting Cells / immunology
  • Antigens, Differentiation, B-Lymphocyte / immunology*
  • Autoantigens / immunology
  • CD4-Positive T-Lymphocytes / immunology
  • Cell Culture Techniques
  • Complement C5 / immunology
  • Histocompatibility Antigens Class II / immunology*
  • Mice
  • Mice, Transgenic
  • Protein Isoforms / immunology
  • Receptors, Antigen, T-Cell / genetics
  • Receptors, Antigen, T-Cell / immunology*
  • Thymus Gland / immunology

Substances

  • Antigens, Differentiation, B-Lymphocyte
  • Autoantigens
  • Complement C5
  • Histocompatibility Antigens Class II
  • Protein Isoforms
  • Receptors, Antigen, T-Cell
  • invariant chain