Chlamydia trachomatis antibody detection and diagnosis of reactive arthritis

Br J Rheumatol. 1998 Oct;37(10):1054-9. doi: 10.1093/rheumatology/37.10.1054.

Abstract

Objective: To investigate whether determining the presence of serum or synovial fluid (SF) IgG and IgA of anti-Chlamydia antibodies with two recent commercially available enzyme-linked immunosorbent assays (ELISA) using synthetic peptides or recombinant antigen could be helpful to detect possible Chlamydia trachomatis (CT)-involved disease in rheumatological patients without evidence of urogenital CT infection.

Methods: The prevalence of such antibodies was determined in samples from patients with well-defined disease, i.e. CT sexually acquired arthritis and from patients with other inflammatory arthropathies unrelated to CT.

Results: When considering IgG and/or IgA anti-MOMP or anti-LPS antibodies, a sensitivity of 100% was obtained for serum and SF samples, but with a low specificity. A sensitivity and a specificity equal or close to 80% were observed for the SF IgG anti-MOMP antibodies.

Conclusion: Clinically, the most appropriate determination was the SF IgG anti-MOMP antibodies. This commercially available ELISA test could be useful for the diagnosis of probable CT reactive arthritis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Antibodies, Bacterial / analysis*
  • Antigens, Bacterial / immunology
  • Arthritis, Reactive / diagnosis*
  • Arthritis, Reactive / immunology
  • Arthritis, Reactive / microbiology
  • Bacterial Outer Membrane Proteins / immunology
  • Chlamydia Infections / diagnosis*
  • Chlamydia Infections / immunology
  • Chlamydia Infections / microbiology
  • Chlamydia trachomatis / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Evaluation Studies as Topic
  • Female
  • Humans
  • Immunoglobulin A / analysis
  • Immunoglobulin G / analysis
  • Male
  • Middle Aged
  • Porins*
  • Predictive Value of Tests
  • Recombinant Proteins
  • Synovial Fluid / immunology
  • Synovial Fluid / microbiology

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Outer Membrane Proteins
  • Immunoglobulin A
  • Immunoglobulin G
  • Porins
  • Recombinant Proteins
  • omp1 protein, Chlamydia trachomatis