Mouse placental cells secrete soluble leptin receptor (sOB-R): cAMP inhibits sOB-R production

Biochem Biophys Res Commun. 1998 Nov 18;252(2):363-7. doi: 10.1006/bbrc.1998.9636.


The aims of this study were to identify whether mouse placenta secretes soluble OB-R (sOB-R) and to find the regulating factor of OB-R expression. Total RNAs were extracted from placenta and decidua, and OB-R expression was assessed by Northern blot analysis. Decidua did not express OB-R mRNA. However, OB-R mRNA expression was detectable in the placenta on day 13 of pregnancy, and then it increased and reached a peak on day 17 of pregnancy. Mouse placental cells from day 12 of pregnancy were cultured and OB-R gene expression was assessed by Northern blot analysis. OB-R mRNA expression was detectable from the second day of culture and reached a peak on the third day of culture. To determine whether placental cells release sOB-R, supernatant of cultured placental cells was subjected to Western blot analysis. sOB-R was detected in the medium by the second day of culture and sOB-R release increased up to the fourth day of culture. Addition of leptin to the medium did not affect expression of OB-R mRNA. However, 8-bromo cAMP inhibited both steady-state levels of OB-R mRNA and the amount of sOB-R protein in the medium in a dose- and time-dependent manner. These results suggest that trophoblast cells differentiate, express, and release sOB-R both in vivo and in vitro and that cAMP is one of several potent regulators of sOB-R secretion by the mouse placenta.

MeSH terms

  • 8-Bromo Cyclic Adenosine Monophosphate / pharmacology
  • Animals
  • Carrier Proteins / biosynthesis
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cells, Cultured
  • Cyclic AMP / metabolism
  • Female
  • Gene Expression / drug effects
  • Leptin
  • Mice
  • Placenta / drug effects
  • Placenta / metabolism*
  • Pregnancy
  • Proteins / metabolism
  • Proteins / pharmacology
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Cell Surface*
  • Receptors, Leptin
  • Solubility


  • Carrier Proteins
  • Leptin
  • Proteins
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Leptin
  • leptin receptor, mouse
  • 8-Bromo Cyclic Adenosine Monophosphate
  • Cyclic AMP