Further complexity of the human SOX gene family revealed by the combined use of highly degenerate primers and nested PCR

FEBS Lett. 1998 Nov 6;438(3):311-4. doi: 10.1016/s0014-5793(98)01294-0.

Abstract

SOX proteins contain a conserved HMG-related DNA-binding domain. They fulfill essential functions during the development of animals. Mutations in several SOX genes have been implicated in human diseases. We present here a new set of PCR primers designed to amplify a broad range of SOX HMG-box sequences. These primers facilitated the cloning of several new SOX HMG boxes from human genomic DNA, revealing unexpected complexity of the SOX gene family.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Conserved Sequence
  • DNA Primers
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics*
  • High Mobility Group Proteins / biosynthesis
  • High Mobility Group Proteins / chemistry
  • High Mobility Group Proteins / genetics*
  • Humans
  • Mammals
  • Mice
  • Molecular Sequence Data
  • Multigene Family*
  • Nuclear Proteins*
  • Phylogeny
  • Polymerase Chain Reaction
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Sex-Determining Region Y Protein
  • Transcription Factors*

Substances

  • DNA Primers
  • DNA-Binding Proteins
  • High Mobility Group Proteins
  • Nuclear Proteins
  • Recombinant Proteins
  • Sex-Determining Region Y Protein
  • Transcription Factors

Associated data

  • GENBANK/AF032449
  • GENBANK/AF032450
  • GENBANK/AF032451
  • GENBANK/AF032452
  • GENBANK/AF032453
  • GENBANK/AF032454