Chromosome landing at the barley Rar1 locus

Mol Gen Genet. 1998 Oct;260(1):92-101. doi: 10.1007/s004380050874.

Abstract

The barley Rar1 gene is an essential component of the race-specific, Mla-12-specified powdery mildew resistance reaction. As part of a map-based cloning strategy designed to isolate Rar1, five barley yeast artificial chromosomes (YACs) have been identified, ranging in size from 300 to 1100 kb. PCR-based YAC end-specific markers have been established and were employed to construct a local YAC contig. Four out of five YAC clones were found to be non-colinear with the source DNA. High-resolution genetic mapping of the YAC ends demonstrated that the set of five overlapping YAC clones encompasses the barley Rar1 gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosomes, Artificial, Yeast
  • Cloning, Molecular
  • Contig Mapping*
  • Electrophoresis, Gel, Pulsed-Field
  • Fungi / pathogenicity
  • Gene Library
  • Genes, Plant*
  • Hordeum / genetics*
  • Plant Diseases / genetics*
  • Polymerase Chain Reaction
  • Recombination, Genetic
  • Selection, Genetic