Electronic PCR: bridging the gap between genome mapping and genome sequencing

Trends Biotechnol. 1998 Nov;16(11):456-9. doi: 10.1016/s0167-7799(98)01232-3.


A crucial event in the history of the Human Genome Project was the decision to use sequence-tagged sites (STSs) as common landmarks for genomic mapping. Following several years of constructing STS-based maps of ever-increasing detail, the emphasis has recently shifted towards large-scale genomic sequencing. A computational procedure called 'electronic PCR' allows STS landmarks to be revealed as data emerge from the sequencing pipeline, thereby bridging the gap between mapping and sequencing activities.

Publication types

  • Review

MeSH terms

  • Base Sequence
  • Chromosome Mapping*
  • Genome, Human
  • Humans
  • Models, Genetic*
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Sequence Analysis, DNA / methods*
  • Sequence Tagged Sites*