Real-time imaging of gene expression in single living cells

Chem Biol. 1998 Nov;5(11):R285-90. doi: 10.1016/s1074-5521(98)90287-3.


Recent advances in reporter gene technologies are now allowing us to image gene transcription at the single cell level, using either fluorescence or luminescence microscopy. Here, the basis of these techniques is outlined and their advantages and disadvantages in various biological systems are discussed.

Publication types

  • Review

MeSH terms

  • Animals
  • Cells / metabolism
  • Cells / ultrastructure*
  • Fluorescent Dyes
  • Gene Expression Regulation / physiology*
  • Humans
  • Image Processing, Computer-Assisted / methods*


  • Fluorescent Dyes