Sequence and phylogenetic position of a class II aldolase gene in the amitochondriate protist, Giardia lamblia

Gene. 1998 Nov 19;222(2):163-8. doi: 10.1016/s0378-1119(98)00499-5.


A Giardia lamblia gene, Glfba, was cloned and sequenced. This gene codes for a 324-residue-long putative class II fructose-1, 6-bisphosphate aldolase. The positions of gaps and phylogenetic analysis with maximum likelihood and maximum parsimony methods showed the sequence to be most closely related to the as-yet uncharacterized aldolases of Helicobacter pylori and Aquifex aeolicus and to the group that comprises the Calvin-cycle aldolases of photosynthetic proteobacteria and cyanobacteria. In combination with the known taxonomic and functional distribution of class I and II aldolases, the results indicate that the G. lamblia enzyme is distinct in its evolutionary history from all eukaryotic fructose-1, 6-bisphosphate aldolases studied so far.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA Primers / genetics
  • DNA, Protozoan / genetics
  • Evolution, Molecular
  • Fructose-Bisphosphate Aldolase / classification
  • Fructose-Bisphosphate Aldolase / genetics*
  • Genes, Protozoan*
  • Giardia lamblia / enzymology*
  • Giardia lamblia / genetics*
  • Helicobacter pylori / enzymology
  • Helicobacter pylori / genetics
  • Molecular Sequence Data
  • Open Reading Frames
  • Phylogeny
  • Sequence Homology, Amino Acid


  • DNA Primers
  • DNA, Protozoan
  • Fructose-Bisphosphate Aldolase

Associated data

  • GENBANK/AF079109