Structure and mapping of the human beta-defensin HBD-2 gene and its expression at sites of inflammation

Gene. 1998 Nov 19;222(2):237-44. doi: 10.1016/s0378-1119(98)00480-6.


We cloned a second human beta-defensin gene, HBD-2, and determined its gene structure and expression in inflamed tissue sections. The entire gene spanned about 2 kb with two small exons and one intron. Radiation hybrid studies confirmed the location on chromosome 8p, were consistent with the order HNP-1, HBD-1 and HBD-2, and located HBD-2 as the most centromeric of the genes. By three-color fluorescence in situ hybridization on both free chromatin fiber mapping and interphase mapping, HBD-1, HBD-2 and HNP-1 were mapped to chromosome 8p23. HBD-1 was within 40-100kb of HNP-1, while HBD-2 was about 500-600 kb from HBD-1, with the most likely order HNP-1, HBD-1, HBD-2. The expression of HBD-2 was locally regulated by inflammation. HBD-2 mRNA was markedly increased in the epidermis surrounding inflamed regions, but not detectable in adjacent non-inflamed areas, a distribution that was confirmed at the peptide level by immunostaining with HBD-2 antibody. The HBD-2 gene is the first member of the human defensin family that is locally inducible by inflammation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Defensins
  • Female
  • Gene Expression
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization, Fluorescence
  • Inflammation / genetics*
  • Inflammation / metabolism
  • Inflammation / pathology
  • Male
  • Molecular Sequence Data
  • Oligonucleotide Probes / genetics
  • Proteins / genetics*
  • Restriction Mapping
  • alpha-Defensins*
  • beta-Defensins*


  • DEFB1 protein, human
  • DEFB4A protein, human
  • DNA, Complementary
  • Defensins
  • Oligonucleotide Probes
  • Proteins
  • alpha-Defensins
  • beta-Defensins
  • human neutrophil peptide 1

Associated data

  • GENBANK/AF040153