Expression of immediate early gene cyr61 during the differentiation of immortalized embryonic hippocampal neuronal cells

Neurosci Lett. 1998 Oct 23;255(3):155-8. doi: 10.1016/s0304-3940(98)00733-2.


Growth factor-mediated signal transduction is a process that is of fundamental importance in understanding cellular growth and differentiation. In order to elucidate the signaling pathways leading to neuronal differentiation, we have tried to identify intermediates that are selectively induced in the differentiation of immortalized neuronal hippocampal cell line H19-7. In the present study we found that immediate early gene cyr61 is expressed in a rapid and transient manner by bFGF during the differentiation of H19-7 cells. To clarify the signal transduction pathway for the induction of cyr61 by bFGF, we checked whether Raf-1 and mitogen-activated protein kinase (MAPK) is activated during the induction of cyr61. It is identified that cyr61 is induced by bFGF via at least two signaling pathways; MAPK-dependent as well as MAPK-independent signaling pathways. This study suggested that cyr61 is likely to play an important role in neuronal differentiation process.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism
  • Cell Differentiation / physiology
  • Cell Line
  • Cysteine-Rich Protein 61
  • Enzyme Activation / physiology
  • Fibroblast Growth Factor 2 / pharmacology
  • Gene Expression / drug effects
  • Gene Expression / physiology*
  • Growth Substances / genetics*
  • Hippocampus / cytology
  • Hippocampus / drug effects
  • Hippocampus / embryology
  • Hippocampus / physiology*
  • Immediate-Early Proteins / genetics*
  • Intercellular Signaling Peptides and Proteins*
  • Neurons / cytology
  • Neurons / drug effects
  • Proto-Oncogene Proteins c-raf / metabolism
  • Rats
  • Signal Transduction / physiology
  • Time Factors


  • CCN1 protein, rat
  • Cysteine-Rich Protein 61
  • Growth Substances
  • Immediate-Early Proteins
  • Intercellular Signaling Peptides and Proteins
  • Fibroblast Growth Factor 2
  • Proto-Oncogene Proteins c-raf
  • Calcium-Calmodulin-Dependent Protein Kinases