Role of IL-1 beta and TNF-alpha in the regulation of NGF in experimentally induced arthritis in mice

Rheumatol Int. 1998;18(3):97-102. doi: 10.1007/s002960050065.


The aim of this study was to investigate the effects of IL-1 beta and TNF-alpha on NGF levels in the knee joint in experimentally induced arthritis in adult mice. Out data showed that IL-1 beta, but not TNF-alpha, induces an increase in NGF levels, while concomitant injection of both cytokines enhances the effect of IL-1 beta on NGF presence. Analysis of NGF levels in the knee joint of carrageenan- and IL-1 beta-induced inflammation after administration of antibodies against TNF-alpha supports this hypothesis. Our studies also showed that exogenous administration of NGF antibody reduces the enhanced level of TNF-alpha occurring in arthritic mice. This latter observation indirectly suggests that NGF is implicated in the upregulation of TNF-alpha in these animal models of joint inflammation. The functional significance of NGF or NGF antibody in inflammatory arthritis is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / pharmacology
  • Arthritis, Experimental / metabolism*
  • Carrageenan / toxicity
  • Interleukin-1 / pharmacology*
  • Interleukin-1 / physiology
  • Knee Joint / drug effects*
  • Knee Joint / metabolism
  • Male
  • Mice
  • Nerve Growth Factors / immunology
  • Nerve Growth Factors / metabolism*
  • Recombinant Proteins / pharmacology
  • Tumor Necrosis Factor-alpha / pharmacology*
  • Tumor Necrosis Factor-alpha / physiology
  • Up-Regulation


  • Antibodies, Monoclonal
  • Interleukin-1
  • Nerve Growth Factors
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • Carrageenan