Interleukin-12 expression in B cells by transformation with Epstein-Barr virus

Biochem Biophys Res Commun. 1998 Nov 27;252(3):556-60. doi: 10.1006/bbrc.1998.9693.


Although interleukin (IL)-12 was originally purified from an Epstein-Barr (EBV)-transformed B cell line and the high correlation of EBV infection and IL-12 expression has been suggested, no study has reported whether EBV infection is directly linked to IL-12 expression. To address this issue, we have investigated IL-12 expression in B cells during in vitro transformation with EBV. Human peripheral B cells became capable of constitutively producing p40 by in vitro transformation with EBV, coincident with the expression of latent membrane protein 1 (LMP1) of EBV. These B cells expressed p40 and p35 mRNA, and phorbol myristate acetate (PMA) stimulation strongly enhanced p40 and p70 production. Furthermore, transfection with LMP1 expression vector into a human B lymphoma cell line, Daudi, led to p40 production with nuclear factor (NF)-kappaB activation. These results suggest that transformation of primary B cells with EBV induces IL-12 expression potentially through LMP1 expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • B-Lymphocytes / metabolism*
  • B-Lymphocytes / virology
  • Burkitt Lymphoma / metabolism
  • Cell Transformation, Viral*
  • Glycoproteins / metabolism
  • Herpesvirus 4, Human / metabolism*
  • Humans
  • Interleukin-12 / biosynthesis*
  • Interleukins
  • Minor Histocompatibility Antigens
  • NF-kappa B / pharmacology
  • Protein Conformation
  • Receptors, Cytokine*
  • Transfection
  • Tumor Cells, Cultured
  • Viral Matrix Proteins / biosynthesis*
  • Viral Matrix Proteins / genetics


  • EBI3 protein, human
  • EBV-associated membrane antigen, Epstein-Barr virus
  • Glycoproteins
  • Interleukins
  • Minor Histocompatibility Antigens
  • NF-kappa B
  • Receptors, Cytokine
  • Viral Matrix Proteins
  • Interleukin-12