TLE, the human homolog of groucho, interacts with AML1 and acts as a repressor of AML1-induced transactivation

Biochem Biophys Res Commun. 1998 Nov 27;252(3):582-9. doi: 10.1006/bbrc.1998.9705.


The AML1 gene encodes DNA-binding proteins that contain the Runt domain and is found at the breakpoints of some translocations associated with leukemias. It has been reported that AML1 plays pivotal roles in myeloid differentiation, probably through the transcriptional regulation of various hematopoietic genes. Here we demonstrate the physical and the functional interaction between AML1 and TLE1 (transducin-like Enhancer of split) human homolog of Groucho that is known to be a corepressor of Hairy-related proteins. TLE1 binds to AML1 through the Runt domain and the C terminus of AML1 that includes the VWRPY motif. The interaction is mainly mediated by the SP domain of TLE1. Moreover, TLE1 inhibits AML1-induced transactivation of the target promoters through the C terminus of AML1. These results suggest that TLE1 acts as a repressor of AML1 and provide important insights into the mechanism of the negative regulation of the AML1 functions in hematopoiesis and leukemogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Basic Helix-Loop-Helix Transcription Factors
  • Binding Sites
  • COS Cells
  • Co-Repressor Proteins
  • Core Binding Factor Alpha 2 Subunit
  • DNA-Binding Proteins / metabolism
  • HeLa Cells
  • Humans
  • Leukocyte Elastase / genetics
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Promoter Regions, Genetic
  • Proto-Oncogene Proteins*
  • Receptor, Macrophage Colony-Stimulating Factor / metabolism
  • Repressor Proteins / metabolism*
  • Transcription Factors / metabolism*
  • Transcriptional Activation*


  • Basic Helix-Loop-Helix Transcription Factors
  • Co-Repressor Proteins
  • Core Binding Factor Alpha 2 Subunit
  • DNA-Binding Proteins
  • Nuclear Proteins
  • Proto-Oncogene Proteins
  • RUNX1 protein, human
  • Repressor Proteins
  • TLE1 protein, human
  • Transcription Factors
  • gro protein, Drosophila
  • Receptor, Macrophage Colony-Stimulating Factor
  • Leukocyte Elastase