Analysis of the ntp1+ gene, encoding neutral trehalase in the fission yeast Schizosaccharomyces pombe

Biochim Biophys Acta. 1998 Nov 26;1443(1-2):225-9. doi: 10.1016/s0167-4781(98)00214-0.


We have cloned and sequenced the ntp1+ gene that codes for neutral trehalase in the fission yeast Schizosaccharomyces pombe. The ntp1+ gene product (Ntp1p) showed a 45-55% identity with neutral trehalases from other yeasts at the amino acid sequence level. However, in clear contrast to other neutral yeast trehalases so far characterized (which show two cAMP phospho-sites), only one consensus site for cAMP-dependent protein phosphorylation was found in Ntp1p. Northern blot hybridization experiments demonstrated that the Wis-Phh1/Sty1 MAP kinase cascade regulates ntp1+ expression during osmostress.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cloning, Molecular
  • Genes, Fungal*
  • Molecular Sequence Data
  • Schizosaccharomyces / enzymology
  • Schizosaccharomyces / genetics*
  • Sequence Alignment
  • Trehalase / genetics*


  • Trehalase

Associated data

  • GENBANK/AJ224339