Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 131 (1), 65-70

Hyaluronidase Reduces the Interstitial Fluid Pressure in Solid Tumours in a Non-Linear Concentration-Dependent Manner

Affiliations

Hyaluronidase Reduces the Interstitial Fluid Pressure in Solid Tumours in a Non-Linear Concentration-Dependent Manner

C Brekken et al. Cancer Lett.

Abstract

Hyaluronidase has gained increasing interest as an adjuvant in local and systemic cancer therapy, despite the incomplete knowledge of its physiological function. To this end, direct intratumoral injection of bovine testicular hyaluronidase (500, 1600 or 7500 U in 50 microl phosphate-buffered saline (PBS)) was performed in orthotopic (o.t.) osteosarcoma xenografts grown in the hind leg of nude mice. Control tumours received 50 microl PBS alone or supplemented with 10% bovine serum albumin (BSA). Central tumour interstitial fluid pressure (IFP) and mean arterial blood pressure (MABP) were measured using the wick-in-needle technique and after cannulation of the carotid artery, respectively. IFP was 32 +/- 8 mmHg (n = 44, mean +/- SD) in untreated tumours and there was a significant correlation between tumour IFP and volume (P < 0.01). The hyaluronidase injection reduced IFP to 63-84% after 1 h compared with controls (P < 0.05) and in a non-linear concentration-dependent manner. MABP was not affected significantly. In conclusion, an intratumoral hyaluronidase injection might reduce IFP temporally in solid osteosarcoma xenografts.

Similar articles

See all similar articles

Cited by 18 PubMed Central articles

See all "Cited by" articles

Publication types

Substances

LinkOut - more resources

Feedback