CD38+ CD45RB(low) CD4+ T cells: a population of T cells with immune regulatory activities in vitro

Eur J Immunol. 1998 Nov;28(11):3435-47. doi: 10.1002/(SICI)1521-4141(199811)28:11<3435::AID-IMMU3435>3.0.CO;2-P.

Abstract

An antibody reactive with CD38 revealed both phenotypic and functional heterogeneity amongst CD45RB(low) cells. Functional analysis of the CD38+ and CD38- fractions showed that the latter contained T cells which responded to recall antigens and produced high levels of cytokine in response to polyclonal stimulation. In contrast, the CD38+ population failed to proliferate or to produce detectable levels of cytokines. Despite appearing unresponsive, the CD38+ population significantly inhibited anti-CD3-induced proliferation and cytokine secretion by the reciprocal CD38- population. Immune suppression required stimulation through the TCR and was dependent on a physical interaction between regulatory and responding CD4+ populations. It did not involve killing of the responding T cells or secretion of IL-10 or TGF-beta. Despite some similarities there is no direct correlation between the in vitro suppression characteristic of the CD38+ CD45RB(low) subset and in vivo suppression which has been shown to be mediated by unseparated CD45RB(low) CD4+ T cells. However, these results demonstrate that two functionally distinct subsets of T cells reside within the antigen-exposed or CD45RB(low) CD4+ T cell population and are thus generated in vivo: (1) conventional memory T cells which proliferate and secrete cytokines in response to activation and (2) a population of regulatory T cells which inhibit T cell activation in vitro. Antibodies reactive with CD38 may provide a useful tool with which to study the role of these T cell subsets in the induction and regulation of the immune response.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADP-ribosyl Cyclase
  • ADP-ribosyl Cyclase 1
  • Animals
  • Antigens, CD*
  • Antigens, Differentiation / analysis*
  • CD4-Positive T-Lymphocytes / immunology*
  • Cell Communication
  • Interleukin-10 / metabolism
  • Interleukin-2 / pharmacology
  • Interleukin-4 / metabolism
  • Leukocyte Common Antigens / analysis*
  • Lymphocyte Activation
  • Membrane Glycoproteins
  • Mice
  • Mice, Inbred BALB C
  • NAD+ Nucleosidase / analysis*
  • Receptors, Antigen, T-Cell / physiology
  • T-Lymphocyte Subsets / immunology*
  • T-Lymphocytes, Regulatory / immunology

Substances

  • Antigens, CD
  • Antigens, Differentiation
  • Interleukin-2
  • Membrane Glycoproteins
  • Receptors, Antigen, T-Cell
  • Interleukin-10
  • Interleukin-4
  • Leukocyte Common Antigens
  • ADP-ribosyl Cyclase
  • Cd38 protein, mouse
  • NAD+ Nucleosidase
  • ADP-ribosyl Cyclase 1