Kid-1 expression is high in differentiated renal proximal tubule cells and suppressed in cyst epithelia

Am J Physiol. 1998 Dec;275(6):F928-37. doi: 10.1152/ajprenal.1998.275.6.F928.

Abstract

The cDNA coding for the transcriptional repressor protein Kid-1 was cloned in a screen for zinc finger proteins, which are regulated during renal development and after renal ischemia. Kid-1 mRNA levels increase in the course of postnatal renal development and decrease after acute renal injury caused by ischemia or administration of folic acid. We have raised a monoclonal anti-Kid-1 antibody and demonstrate that the Kid-1 protein is strongly expressed in the proximal tubule of the adult rat kidney. During nephron development, the Kid-1 protein appears after the S-shaped body stage concomitantly with the brush-border enzyme alkaline phosphatase. In two animal models of polycystic kidney disease, the expression of Kid-1 is downregulated. The loss of expression of Kid-1 in cyst wall cells correlates with the loss of alkaline phosphatase histochemical staining. Kid-1 mRNA levels are also reduced in rodent renal cell carcinomas, another condition characterized by epithelial cell dedifferentiation and increased proliferation. We propose that Kid-1 plays an important role during the differentiation of the proximal tubule.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aging / metabolism
  • Animals
  • Animals, Newborn / metabolism
  • Antibodies, Monoclonal
  • COS Cells
  • Carcinoma, Renal Cell / metabolism
  • Carcinoma, Renal Cell / pathology
  • Cell Line, Transformed / metabolism
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Epithelial Cells / metabolism
  • Epithelium / metabolism
  • Immunohistochemistry / methods
  • Kidney / cytology
  • Kidney / metabolism
  • Kidney Neoplasms / metabolism
  • Kidney Neoplasms / pathology
  • Kidney Tubules, Proximal / cytology
  • Kidney Tubules, Proximal / metabolism*
  • Mice
  • Polycystic Kidney Diseases / metabolism*
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Transcription Factors*

Substances

  • Antibodies, Monoclonal
  • DNA-Binding Proteins
  • RNA, Messenger
  • Transcription Factors
  • Zfp354a protein, mouse
  • Zfp354a protein, rat