Identification of a proline-binding motif regulating CD2-triggered T lymphocyte activation

Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14897-902. doi: 10.1073/pnas.95.25.14897.

Abstract

An intracellular protein termed CD2 binding protein 2 (CD2BP2), which binds to a site containing two PPPGHR segments within the cytoplasmic region of CD2, was identified. Mutagenesis and NMR analysis demonstrated that the CD2 binding region of CD2BP2 includes a 17-aa motif (GPY[orF]xxxxM[orV]xxWxxx GYF), also found in several yeast and Caenorhabditis elegans proteins of unknown function. In Jurkat T cells, over-expression of the isolated CD2BP2 domain binding to CD2 enhances the production of interleukin 2 on crosslinking of CD2 but not the T cell receptor. Hence, a proline-binding module distinct from SH3 and WW domains regulates protein-protein interactions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • CD2 Antigens / genetics
  • CD2 Antigens / immunology*
  • Caenorhabditis elegans
  • Carrier Proteins / genetics
  • Carrier Proteins / immunology*
  • DNA Mutational Analysis
  • Humans
  • Lymphocyte Activation*
  • Molecular Sequence Data
  • Proline
  • Saccharomyces cerevisiae
  • Sequence Analysis
  • T-Lymphocytes / immunology*

Substances

  • Adaptor Proteins, Signal Transducing
  • CD2 Antigens
  • CD2BP2 protein, human
  • Carrier Proteins
  • Proline

Associated data

  • GENBANK/AF104222