Arrangement of subunits in 20 S particles consisting of NSF, SNAPs, and SNARE complexes

Mol Cell. 1998 Nov;2(5):539-48. doi: 10.1016/s1097-2765(00)80153-7.

Abstract

The structure of 20 S particles, consisting of NSF, SNAPs, and SNARE complexes, was analyzed by electron microscopy and fluorescence resonance energy transfer. Structural changes associated with the binding of alpha-SNAP and NSF to SNARE complexes define the contribution of each component to the 20 S particle structure. The synaptic SNARE complex forms a 2.5 x 15 nm rod. alpha-SNAP binds laterally to the rod, increasing its width but not its length. NSF binds to one end of the SNAP/SNARE complex; the resulting 20 S particles measure 22 nm in length and vary in width from 6 nm at their narrowest point to 13.5 nm at their widest. The transmembrane domains of VAMP and syntaxin emerge together at the NSF-distal end of 20 S particles, adjacent to the amino terminus of alpha-SNAP.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Animals
  • Brain
  • Carrier Proteins / chemistry
  • Carrier Proteins / isolation & purification
  • Carrier Proteins / metabolism*
  • Carrier Proteins / ultrastructure
  • Centrifugation, Density Gradient
  • Dimerization
  • Energy Transfer
  • Escherichia coli / genetics
  • Fluorometry
  • Glutathione Transferase / metabolism
  • Luminescent Proteins / metabolism
  • Membrane Proteins / chemistry
  • Membrane Proteins / isolation & purification
  • Membrane Proteins / metabolism*
  • Membrane Proteins / ultrastructure
  • Microscopy, Immunoelectron
  • N-Ethylmaleimide-Sensitive Proteins
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / metabolism
  • Nerve Tissue Proteins / ultrastructure
  • Protein Binding
  • Protein Conformation
  • Proto-Oncogene Proteins c-myc / immunology
  • Qa-SNARE Proteins
  • R-SNARE Proteins
  • Rats
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / metabolism
  • SNARE Proteins
  • Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
  • Vesicular Transport Proteins*

Substances

  • Carrier Proteins
  • Luminescent Proteins
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Proto-Oncogene Proteins c-myc
  • Qa-SNARE Proteins
  • R-SNARE Proteins
  • Recombinant Fusion Proteins
  • SNARE Proteins
  • Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
  • Vesicular Transport Proteins
  • Adenosine Triphosphate
  • Glutathione Transferase
  • N-Ethylmaleimide-Sensitive Proteins
  • Nsf protein, rat