Histone-like TAFs are essential for transcription in vivo

Mol Cell. 1998 Nov;2(5):663-73. doi: 10.1016/s1097-2765(00)80164-1.

Abstract

In yeast, the TBP-associated factors (TAFs) Taf17, Taf60, and Taf61(68) resemble histones H3, H4, and H2B, respectively. To analyze their roles in vivo, conditional alleles were isolated by mutagenizing their histone homology domains. Conditional alleles of TAF17 or TAF60 can be specifically suppressed by overexpression of any of the other histone-like TAFs. This and other genetic evidence supports the model of a histone octamer-like structure within TFIID. Shifting strains carrying the conditional TAF alleles to non-permissive conditions results in degradation of TFIID components and the rapid loss of mRNA production. Therefore, in contrast to previous studies in yeast that found only limited roles for TAFs in transcription, we find that the histone-like TAFs are generally required for in vivo transcription.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Alcohol Oxidoreductases
  • Alleles
  • Amino Acid Sequence
  • Aminohydrolases
  • Cell Division
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Enzyme Stability
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Genes, Fungal / genetics
  • Histones / genetics
  • Molecular Sequence Data
  • Multienzyme Complexes
  • Mutagenesis
  • Phenotype
  • Pyrophosphatases
  • RNA Polymerase II / metabolism
  • RNA, Messenger / analysis
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • Suppression, Genetic / genetics
  • TATA-Binding Protein Associated Factors*
  • Trans-Activators / genetics
  • Trans-Activators / metabolism
  • Transcription Factor TFIID
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcription Factors, TFII / genetics
  • Transcription Factors, TFII / metabolism*
  • Transcription, Genetic*

Substances

  • ADA2 protein, S cerevisiae
  • Adaptor Proteins, Signal Transducing
  • DNA-Binding Proteins
  • Fungal Proteins
  • HFI1 protein, S cerevisiae
  • Histones
  • Multienzyme Complexes
  • RNA, Messenger
  • Saccharomyces cerevisiae Proteins
  • TAF(II)61 protein, S cerevisiae
  • TAF6 protein, S cerevisiae
  • TAF9 protein, S cerevisiae
  • TATA-Binding Protein Associated Factors
  • Trans-Activators
  • Transcription Factor TFIID
  • Transcription Factors
  • Transcription Factors, TFII
  • Alcohol Oxidoreductases
  • HIS4 protein, S cerevisiae
  • RNA Polymerase II
  • Aminohydrolases
  • Pyrophosphatases