A 1,3-beta-glucanosyltransferase isolated from the cell wall of Aspergillus fumigatus is a homologue of the yeast Bgl2p

Microbiology (Reading). 1998 Nov;144 ( Pt 11):3171-80. doi: 10.1099/00221287-144-11-3171.

Abstract

A 1,3-beta-glucanosyltransferase which introduces intrachain 1,6-beta linkages into 1,3-beta-glucan was isolated from the cell wall of Aspergillus fumigatus. The biochemical and molecular characterization of the A. fumigatus transferase showed it was homologous to the Saccharomyces cerevisiae and Candida albicans transferase Bgl2p. A null mutant constructed in A. fumigatus by gene replacement did not show a distinct phenotype from the parental strain. The putative function of this major cell-wall-associated protein is discussed.

MeSH terms

  • Amino Acid Sequence
  • Aspergillus fumigatus / enzymology*
  • Aspergillus fumigatus / genetics
  • Candida albicans / enzymology
  • Candida albicans / genetics
  • Cell Wall / enzymology*
  • Chromatography, Ion Exchange
  • Cloning, Molecular
  • DNA, Fungal / analysis
  • Genes, Fungal
  • Glucan Endo-1,3-beta-D-Glucosidase / chemistry
  • Glucan Endo-1,3-beta-D-Glucosidase / genetics
  • Glucan Endo-1,3-beta-D-Glucosidase / isolation & purification
  • Glucan Endo-1,3-beta-D-Glucosidase / metabolism*
  • Molecular Sequence Data
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins*
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Transformation, Genetic

Substances

  • DNA, Fungal
  • Saccharomyces cerevisiae Proteins
  • 1,3-beta-glucanosyltransferase
  • BGL2 protein, S cerevisiae
  • Glucan Endo-1,3-beta-D-Glucosidase

Associated data

  • GENBANK/AF038596