Chitin-graft-poly(2-methyl-2-oxazoline) enhanced solubility and activity of catalase in organic solvent

Int J Biol Macromol. 1998 Nov;23(4):259-62. doi: 10.1016/s0141-8130(98)00055-5.

Abstract

Catalase from bovine liver was lyophilized from an aqueous solution containing chitin-graft-poly(2-methyl-2-oxazoline) (3), which was synthesized by the reaction of 52% deacetylated chitin (1) with living poly(2-methyl-2-oxazoline) (2). The rate of consumption of H2O2 in chloroform catalyzed by the lyophilized catalase with 3 was enhanced more than 10 times that by catalase without 3. The dispersibility and solubility of lyophilized catalase with 3 in chloroform were improved in comparison with catalase itself.

MeSH terms

  • Animals
  • Catalase / chemistry
  • Catalase / isolation & purification
  • Catalase / metabolism*
  • Cattle
  • Chitin / analogs & derivatives*
  • Chitin / chemical synthesis
  • Chitin / chemistry*
  • Chitin / metabolism*
  • Chloroform / chemistry
  • Hydrogen Peroxide / chemistry
  • Hydrogen Peroxide / metabolism
  • Liver / enzymology
  • Organic Chemicals / chemistry
  • Polyamines
  • Solubility
  • Solvents

Substances

  • Organic Chemicals
  • Polyamines
  • Solvents
  • poly(2-methyl-2-oxazoline)
  • Chitin
  • Chloroform
  • Hydrogen Peroxide
  • Catalase