Thyroid transcription factor-1 and cytokeratins 7 and 20 in pulmonary and breast carcinoma

Acta Cytol. Nov-Dec 1998;42(6):1382-8. doi: 10.1159/000332172.

Abstract

Objective: To evaluate the immunohistochemical expression of a lung epithelial gene transcription factor, thyroid transcription factor-1 (TTF-1), in lung and breast carcinoma in pulmonary cytologic preparations and to correlate the results with the expression of cytokeratin 7 (CK7) and 20 (CK20).

Study design: Cell blocks of cytologic specimens were immunostained with antibodies to TTF-1, CK7 and CK20. Specimens included 41 primary lung carcinomas (21 adenocarcinomas, 8 squamous cell carcinomas and 12 small cell undifferentiated carcinomas) and 6 metastatic breast adenocarcinomas.

Results: The lung adenocarcinomas showed nuclear reactivity for TTF-1 in 76% (16/21) of the cases and a staining combination of CK7+/CK20- in 95% (20/21) of the cases. Only one case was CK7+/CK20+. All the breast carcinomas were nonreactive to TTF-1, and all were CK7+/CK20-. The squamous cell carcinomas and small cell undifferentiated carcinomas showed TTF-1 positivity in 38% (3/8) and 83% (10/12), respectively.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenocarcinoma / metabolism
  • Adenocarcinoma / pathology*
  • Biomarkers, Tumor
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology*
  • Carcinoma, Small Cell / metabolism
  • Carcinoma, Small Cell / pathology
  • Carcinoma, Squamous Cell / metabolism
  • Carcinoma, Squamous Cell / pathology
  • Female
  • Homeodomain Proteins / analysis
  • Humans
  • Intermediate Filament Proteins / analysis*
  • Keratin-20
  • Keratin-7
  • Keratins / analysis*
  • Lung Neoplasms / metabolism
  • Lung Neoplasms / pathology*
  • Nuclear Proteins / analysis*
  • Thyroid Gland / chemistry
  • Thyroid Gland / pathology
  • Thyroid Nuclear Factor 1
  • Transcription Factors / analysis*

Substances

  • Biomarkers, Tumor
  • Homeodomain Proteins
  • Intermediate Filament Proteins
  • KRT20 protein, human
  • KRT7 protein, human
  • Keratin-20
  • Keratin-7
  • NKX2-1 protein, human
  • Nuclear Proteins
  • Thyroid Nuclear Factor 1
  • Transcription Factors
  • Keratins