High levels of transcription factor RpoS (sigma S) in mviA mutants negatively affect 1,2-propanediol-dependent transcription of the cob/pdu regulon of Salmonella typhimurium LT2

FEMS Microbiol Lett. 1998 Dec 1;169(1):147-53. doi: 10.1111/j.1574-6968.1998.tb13311.x.

Abstract

Expression of the cobalamin biosynthetic (cob) and 1,2-propanediol utilization (cob/pdu) regulon of Salmonella typhimurium LT2 is controlled at the transcriptional level by global and specific regulatory proteins. In this paper we show that mutations in the mviA gene negatively affect cob/pdu transcription in response to 1,2-propanediol in the environment. The effects of mviA mutations were consistent with its role in the regulation of RpoS levels in the cell. Null mutations in rpoS eliminated the negative effect of mviA mutations on cob/pdu transcription, and restored growth on succinate, propionate and 1,2-propanediol. In addition, mviA mutants were deficient in the utilization of succinate, propionate and 1,2-propanediol as carbon and energy sources.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism*
  • DNA-Binding Proteins*
  • Escherichia coli Proteins*
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Phenotype
  • Propylene Glycol / metabolism*
  • Regulon
  • Salmonella typhimurium / genetics*
  • Salmonella typhimurium / growth & development
  • Sigma Factor / metabolism*
  • Transcription Factors*
  • Transcription, Genetic
  • Vitamin B 12 / biosynthesis*

Substances

  • Bacterial Proteins
  • DNA-Binding Proteins
  • Escherichia coli Proteins
  • Sigma Factor
  • Transcription Factors
  • rssB protein, E coli
  • sigma factor KatF protein, Bacteria
  • Propylene Glycol
  • Vitamin B 12