Increased Telomerase Activity in Intrahepatic Cholangiocellular Carcinomas Induced by N-nitrosobis(2-oxopropyl)amine in Hamsters

Cancer Lett. 1998 Sep 25;131(2):185-90. doi: 10.1016/s0304-3835(98)00148-7.

Abstract

Telomerase activities in intrahepatic cholangiocarcinomas induced by N-nitrosobis(2-oxopropyl)amine (BOP) in female hamsters were determined using a telomeric repeat amplification protocol (TRAP) assay followed by densitometric quantification. Each determination was repeated to confirm the results and telomerase activity was also detected by gel electrophoresis. An increase was evident in all of 10 cholangiocarcinomas examined, with levels ranging from 2.48 to 4.40 times the normal liver value by densitometric quantification. This finding of a consistent increase suggests that telomerase activation is involved in the development of intrahepatic cholangiocarcinomas and immortalization of cancer cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / metabolism
  • Animals
  • Bile Duct Neoplasms / chemically induced
  • Bile Duct Neoplasms / enzymology*
  • Bile Ducts, Intrahepatic
  • Carcinogens
  • Cholangiocarcinoma / chemically induced
  • Cholangiocarcinoma / enzymology*
  • Cricetinae
  • Cricetulus
  • Female
  • Nitrosamines
  • Telomerase / metabolism*

Substances

  • Carcinogens
  • Nitrosamines
  • nitrosobis(2-oxopropyl)amine
  • Telomerase