Sucrose-phosphate synthase from Synechocystis sp. strain PCC 6803: identification of the spsA gene and characterization of the enzyme expressed in Escherichia coli

J Bacteriol. 1998 Dec;180(24):6776-9. doi: 10.1128/JB.180.24.6776-6779.1998.


The first identification and characterization of a prokaryotic gene (spsA) encoding sucrose-phosphate synthase (SPS) is reported for Synechocystis sp. strain PCC 6803, a unicellular non-nitrogen-fixing cyanobacterium. Comparisons of the deduced amino acid sequence and some relevant biochemical properties of the enzyme with those of plant SPSs revealed important differences in the N-terminal and UDP-glucose binding site regions, substrate specificities, molecular masses, subunit compositions, and regulatory properties.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cloning, Molecular
  • Cyanobacteria / enzymology*
  • Cyanobacteria / genetics
  • Escherichia coli
  • Gene Expression
  • Genes, Bacterial
  • Glucosyltransferases / genetics*
  • Glucosyltransferases / metabolism
  • Molecular Sequence Data
  • Mutagenesis
  • Open Reading Frames
  • Sequence Analysis


  • Glucosyltransferases
  • sucrose-phosphate synthase