Molecular cloning of XNLRR-1, a Xenopus homolog of mouse neuronal leucine-rich repeat protein expressed in the developing Xenopus nervous system

Gene. 1998 Oct 9;221(1):159-66. doi: 10.1016/s0378-1119(98)00414-4.

Abstract

We report the isolation and characterization of a Xenopus sequence, XNLRR-1, that is closely related to a gene for mouse neuronal leucine-rich repeat protein (NLRR-1). The cDNA clone is 4179 bp long and encodes a putative transmembrane glycoprotein of 718 amino acids, containing 12 leucine-rich repeats followed by one C2-type immunoglobulin-like domain and one fibronectin type-III repeat. XNLRR-1 is transcribed mainly in the developing eye area and the ventricular zone from diencephalon to hindbrain and slightly in spinal cord in Xenopus tadpoles. The similarity of the XNLRR-1 gene to other known cell adhesion molecules, together with the expression pattern, suggests that XNLRR-1 is involved in interactions at the neuronal cell surface.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Cloning, Molecular
  • Complement C2
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • Diencephalon / chemistry
  • Diencephalon / embryology
  • Embryo, Nonmammalian / chemistry
  • Embryo, Nonmammalian / metabolism
  • Eye / chemistry
  • Eye / embryology
  • Fibronectins
  • Gene Expression Regulation, Developmental
  • Immunoglobulins / chemistry
  • Membrane Proteins
  • Mice
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics*
  • Nervous System / chemistry
  • Nervous System / embryology
  • Nervous System / metabolism*
  • Protein Conformation
  • Proteins / chemistry
  • Proteins / genetics
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Repetitive Sequences, Amino Acid
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rhombencephalon / chemistry
  • Rhombencephalon / embryology
  • Sequence Homology, Amino Acid
  • Spinal Cord / chemistry
  • Spinal Cord / embryology
  • Tissue Distribution
  • Xenopus / embryology
  • Xenopus / genetics*

Substances

  • Complement C2
  • DNA, Complementary
  • Fibronectins
  • Immunoglobulins
  • Lrrn1 protein, mouse
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Proteins
  • RNA, Messenger
  • leucine-rich repeat proteins

Associated data

  • GENBANK/AB014462