Abstract
Two nested PCRs for the detection of Mycobacterium ulcerans were compared by using a collection of 65 clinical specimens. The first method amplifies the gene coding for 16S rRNA, and the second method amplifies a repetitive DNA sequence. The sensitivities of bacterioscopy, culture, 16S rRNA gene PCR, and repetitive-sequence PCR were 29, 34, 80, and 85%, respectively. Compared to the 16S rRNA gene PCR, the repetitive-sequence PCR was faster, easier to perform, and less expensive.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteriological Techniques
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DNA Transposable Elements / genetics
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DNA, Bacterial / analysis
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DNA, Bacterial / genetics
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Humans
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Mycobacterium ulcerans / genetics
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Mycobacterium ulcerans / isolation & purification*
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Polymerase Chain Reaction / methods*
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RNA, Ribosomal, 16S / analysis
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RNA, Ribosomal, 16S / genetics
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Sensitivity and Specificity
Substances
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DNA Transposable Elements
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DNA, Bacterial
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RNA, Ribosomal, 16S