In situ RT-PCR detection of Epstein-Barr virus immediate-early transcripts in CD4+ and CD8+ T lymphocytes

Anticancer Res. Sep-Oct 1998;18(5A):3171-80.

Abstract

AIDS-related Epstein-Barr virus (EBV)-associated T cell lymphomas are emerging as a new, distinct histopathological entity. The pathway whereby EBV infects T cells as well as the initial EBV transcriptional program in T cells has not been established. In order to shed light on the early events of the EBV infection of T cells, we have used in situ reverse transcription based polymerase chain reaction (RT-PCR) to study the initial EBV transcriptional program in homogeneous CD4+ and CD8+ lymphocytes. Following EBV infection, Epstein-Barr nuclear antigen (EBNA) expression could be detected in T rosetting CD4+ and CD8+ T lymphocytes. Only a few cells showed viral capsid antigen (VCA). EBV immediate-early gene transcripts (BZLF1, BRLF1, and BMLF1) encoded in the BamHI Z, R, and M fragments could be detected by in situ RT-PCR in the EBV producer cell line B95.8. Both BZLF1 and BRLF1 immediate-early transcripts, but not BMLF1 transcript, could be detected in individual CD4+ and CD8+ T cells infected with EBV. Demonstration of EBV mRNA transcripts encoding immediate-early transcriptional transactivators in EBV-infected T cells provides the first evidence for a possible mechanism whereby EBV could contribute to T cell proliferation and EBV-associated T cell malignancies.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, Viral / metabolism*
  • Burkitt Lymphoma / virology
  • CD4-Positive T-Lymphocytes / virology*
  • CD8-Positive T-Lymphocytes / virology*
  • Cell Nucleus / metabolism*
  • DNA-Binding Proteins / metabolism*
  • Herpesvirus 4, Human / metabolism*
  • Humans
  • Immediate-Early Proteins / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Trans-Activators / metabolism*
  • Transcription Factors / metabolism*
  • Viral Proteins*

Substances

  • Antigens, Viral
  • BRLF1 protein, Human herpesvirus 4
  • BZLF1 protein, Herpesvirus 4, Human
  • DNA-Binding Proteins
  • Immediate-Early Proteins
  • Trans-Activators
  • Transcription Factors
  • Viral Proteins