Gene expression of cyclooxygenase-2, group II and cytosolic phospholipase A2 in human colorectal cancer

Anticancer Res. Sep-Oct 1998;18(5A):3283-7.

Abstract

The development of intestinal tumours in the Min mouse is influenced by Moml locus, where the group II phospholipase A2 (PLA2-II) gene has been suggested as the candidate gene. Since no causative genetic alterations have been identified in PLA2-II in human colorectal tumours, we tested whether PLA2-II or the cytosolic isoform cPLA2, are dysregulated in human colorectal carcinogenesis. By means of RT-PCR, the expression of these genes and cyclooxygenase-2 (COX-2), which is upregulated in colorectal tumours, were examined. No changes in gene expression of PLA2-II were found in 44 tumours compared to normal mucosa. cPLA2 and COX-2 were upregulated; however, no correlation in expression was found between these genes. Thus, the supply of arachidonic acid provided by PLA2-II/cPLA2 seems not to be the rate limiting step in PGE2 (a prostaglandin/mitogen) formation via COX-2 and PLA2-II plays a minor or no role in human colorectal carcinogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Colorectal Neoplasms / enzymology*
  • Colorectal Neoplasms / genetics
  • Cyclooxygenase 2
  • Cytoplasm / enzymology
  • Female
  • Group II Phospholipases A2
  • Humans
  • Isoenzymes / genetics
  • Isoenzymes / metabolism*
  • Male
  • Membrane Proteins
  • Middle Aged
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / metabolism*
  • Phospholipases A / genetics
  • Phospholipases A / metabolism*
  • Phospholipases A2
  • Prostaglandin-Endoperoxide Synthases / genetics
  • Prostaglandin-Endoperoxide Synthases / metabolism*
  • RNA, Messenger / metabolism

Substances

  • Isoenzymes
  • Membrane Proteins
  • Neoplasm Proteins
  • RNA, Messenger
  • Cyclooxygenase 2
  • PTGS2 protein, human
  • Prostaglandin-Endoperoxide Synthases
  • Phospholipases A
  • Group II Phospholipases A2
  • Phospholipases A2