The P histo-blood group-related glycosphingolipid sialosyl galactosyl globoside as a preferred binding receptor for uropathogenic Escherichia coli: isolation and structural characterization from human kidney

Biochemistry. 1998 Dec 15;37(50):17420-8. doi: 10.1021/bi9814639.

Abstract

The P histo-blood group-related glycosphingolipid, sialosyl galactosyl globoside (SGG), has recently been implicated as a preferred binding receptor for uropathogenic Escherichia coli [Stapleton, A. E., Stroud, M. R., Hakomori, S., and Stamm, W. E. (1998) Infect. Immun. 66, 3856-3861]. We report here the purification and complete structural characterization of SGG from normal human kidney. Using metabolically [35S]-labeled E. coli as a probe, a monosialylated glycosphingolipid was isolated to homogeneity. The glycosphingolipid was purified by a combination of high-performance liquid chromatography and preparative high-performance thin-layer chromatography and its structure unambiguously elucidated by 1H NMR, electrospray ionization mass spectrometry, and methylation analysis. Its primary structure was shown to be identical to a previously characterized, developmentally regulated, globo-series glycolipid thought to be unique to human teratocarcinoma. The significance of this structure as a unique receptor in human kidney for uropathogenic E. coli and its role in the pathogenesis of urinary tract infections are discussed.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Escherichia coli / metabolism*
  • Escherichia coli / pathogenicity
  • Escherichia coli Infections / etiology
  • Escherichia coli Infections / metabolism*
  • Gangliosides / chemistry
  • Gangliosides / isolation & purification*
  • Gangliosides / metabolism
  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Kidney / chemistry*
  • Kidney / metabolism
  • Mass Spectrometry
  • Methylation
  • Molecular Sequence Data
  • Nuclear Magnetic Resonance, Biomolecular
  • P Blood-Group System / chemistry
  • P Blood-Group System / metabolism*
  • Receptors, Cell Surface / chemistry
  • Receptors, Cell Surface / isolation & purification*
  • Receptors, Cell Surface / metabolism
  • Urinary Tract Infections / etiology
  • Urinary Tract Infections / metabolism*

Substances

  • Gangliosides
  • P Blood-Group System
  • Receptors, Cell Surface
  • glycolipid receptor
  • disialosyl galactosyl globoside