Evaluation of 9-cis retinoic acid for a new remedy of human retinoblastoma

Biochem Mol Biol Int. 1998 Dec;46(5):987-91. doi: 10.1080/15216549800204542.

Abstract

We investigated the effect of two isomers of retinoic acid (RA), all-trans RA and 9-cis RA, on the proliferation of Y79 human retinoblastoma cells. The two isomers inhibited the cell proliferation in a concentration-dependent manner. The IC50 for this inhibition by all-trans RA and 9-cis RA was 1.50 and 0.15 microM, respectively. The inhibitory effect of 9-cis RA on Y79 cell growth was observed within 24 hr, thereafter the cell number was gradually decreased. In contrast, no inhibition by all-trans RA of Y79 cell growth was observed within 24 hr, thereafter the cell number was slightly increased. In these cases, the cell viability at 4 days after the addition of 9-cis RA and all-trans RA was more than 90% and 95%, respectively. These results indicate that the two RA inhibit the proliferation of Y79 human retinoblastoma cells without inducing the cell death and that the effect of 9-cis RA on the inhibition of Y79 cell growth is much greater than that of all-trans RA.

MeSH terms

  • Alitretinoin
  • Antineoplastic Agents / pharmacology*
  • Antineoplastic Agents / therapeutic use
  • Cell Division / drug effects
  • Cell Survival / drug effects
  • Dose-Response Relationship, Drug
  • Humans
  • Inhibitory Concentration 50
  • Retinoblastoma / drug therapy*
  • Retinoblastoma / pathology
  • Time Factors
  • Tretinoin / pharmacology*
  • Tretinoin / therapeutic use
  • Trypan Blue
  • Tumor Cells, Cultured

Substances

  • Antineoplastic Agents
  • Alitretinoin
  • Tretinoin
  • Trypan Blue