Abstract
Inhibitory mechanism of the water extract of Scutellariae Radix on prostaglandin E2 (PGE2) release was examined in C6 rat glioma cells. Scutellariae Radix reduced a Ca2+ ionophore A23187-induced PGE2 release by inhibition of arachidonic acid (AA) liberation. Sho-saiko-to and San'o-shashin-to, which contain Scutellariae Radix, also inhibited PGE2 release. A23187 caused phosphorylation of mitrogen-activated protein kinase (MAPK), resulting in activation of cytosolic phospholipase A2 (cPLA2). Scutellariae Radix and baicalein inhibited the phosphorylation of MAPK. Baicalein, but not baicalin, inhibited A23187-induced PGE2 release. These results suggest that baicalein in Scutellariae Radix reduces AA liberation through the inhibition of the MAPK-cPLA2 pathway.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Antineoplastic Agents / pharmacology*
-
Arachidonic Acid / metabolism
-
Calcimycin / pharmacology
-
Calcium-Calmodulin-Dependent Protein Kinases / metabolism
-
Dinoprostone / antagonists & inhibitors*
-
Dinoprostone / biosynthesis*
-
Drugs, Chinese Herbal / pharmacology*
-
Enzyme Inhibitors / pharmacology*
-
Flavanones*
-
Flavonoids / pharmacology*
-
Glioma / drug therapy
-
Glioma / metabolism*
-
Ionophores / pharmacology
-
Molecular Sequence Data
-
Phosphorylation / drug effects
-
Prostaglandin Antagonists / pharmacology*
-
Rats
-
Tumor Cells, Cultured
Substances
-
Antineoplastic Agents
-
Drugs, Chinese Herbal
-
Enzyme Inhibitors
-
Flavanones
-
Flavonoids
-
Ionophores
-
Prostaglandin Antagonists
-
Arachidonic Acid
-
baicalin
-
Calcimycin
-
baicalein
-
5,7-dihydroxy-6-methoxy-2-phenylchromen-4-one
-
saiko-keishi-to
-
Calcium-Calmodulin-Dependent Protein Kinases
-
Dinoprostone
-
wogonin
-
2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one