Uptake and incorporation of choline and ethanolamine into lipoteichoic acid and teichoic acid by the choline-independent mutant JY2190 of Streptococcus pneumoniae

FEMS Microbiol Lett. 1998 Dec 15;169(2):355-9. doi: 10.1111/j.1574-6968.1998.tb13340.x.

Abstract

Lipoteichoic acid- and teichoic acid-containing muropeptides were isolated from choline- or ethanolamine-grown cells of the choline-independent mutant JY2190 of Streptococcus pneumoniae. Choline was taken up and incorporated into lipoteichoic acid and teichoic acid with 81% efficiency, compared with the parent strain Rx1. With similar efficiency, ethanolamine was incorporated. Accordingly, the mutant is a valuable tool for identifying the individual genes encoding the enzymes of choline utilisation, because any of these genes can be deleted without affecting viability and growth rate.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Choline / genetics
  • Choline / metabolism*
  • Chromatography, Affinity
  • Ethanolamine / metabolism*
  • Lipopolysaccharides / metabolism
  • Mutation
  • Streptococcus pneumoniae / genetics
  • Streptococcus pneumoniae / metabolism*
  • Teichoic Acids / metabolism*

Substances

  • Lipopolysaccharides
  • Teichoic Acids
  • lipoteichoic acid
  • Ethanolamine
  • Choline