Visualization of the Brassica self-incompatibility S-locus on identified oilseed rape chromosomes

Plant Mol Biol. 1998 Dec;38(6):1081-7. doi: 10.1023/a:1006036100987.


Seventy years after Karpechenko [15] first reported the accurate chromosome number of oilseed rape (Brassica napus L., 2n=38), we have developed a quantitative chromosome map of rape using computer imaging technology. The capacity to identify individual rape chromosomes will facilitate a wide range of genetic studies. Here we demonstrate the use of imaging methods in combination with fluorescence in situ hybridization to localize, on identified chromosomes, the single copy S-locus glycoprotein and S-locus-related genes involved in the self-incompatibility system of Brassica. These techniques have a broader application in plant genome research involving the mapping of single-copy genes and markers, irrespective of the plant species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brassica / genetics*
  • Chromosome Mapping*
  • DNA, Ribosomal / genetics
  • Haploidy
  • Image Processing, Computer-Assisted
  • In Situ Hybridization, Fluorescence
  • Karyotyping
  • Plant Roots
  • RNA, Plant / genetics
  • RNA, Ribosomal / genetics*
  • RNA, Ribosomal, 18S / genetics
  • RNA, Ribosomal, 5S / genetics


  • DNA, Ribosomal
  • RNA, Plant
  • RNA, Ribosomal
  • RNA, Ribosomal, 18S
  • RNA, Ribosomal, 5S
  • RNA, ribosomal, 25S