In vivo efficacy of antimicrobial-coated fabric from prosthetic heart valve sewing rings

J Heart Valve Dis. 1998 Nov;7(6):639-46.


Background and aims of the study: Antimicrobial coating of medical devices has recently emerged as a potentially effective method for preventing device-related infections. The objective of this animal study was to examine in vivo the antimicrobial efficacy of prosthetic heart valve sewing ring fabric coated with: (i) silver; (ii) combined minocycline and rifampin (M/R); or (iii) combined chlorhexidine and chloroxylenol (CH/CX).

Methods: A rabbit model of Staphylococcus aureus colonization and infection of subcutaneously implanted fabric of prosthetic heart valve sewing rings was used. Following administration of anesthesia and preoperative antibiotic prophylaxis, 0.5 x 0.5 cm samples of fabric were placed subcutaneously into the back of rabbits. Each rabbit received a total of eight samples: (i) two uncoated; (ii) two silver-coated; (iii) two M/R-coated; and (iv) two CH/CX-coated. After injecting a bacterial inoculum of 2 x 10(5) c.f.u. of S. aureus onto each implanted sample, the wounds were sutured. Rabbits were monitored daily for one week, killed and the test fabrics removed and cultured.

Results: Rates of device colonization, device-related infection and device-related abscess were similar between the uncoated and silver-coated devices. Devices coated with M/R were less likely to be colonized or cause device-related infection when compared with uncoated devices, and less likely to be associated with abscess formation than silver-coated devices. There was a tendency for CH/CX-coated devices to be less colonized than uncoated devices. Only M/R-coated and CH/CX-coated devices produced zones of inhibition in vitro. Implantation of M/R-coated and CH/CX-coated devices in rabbits did not result in detectable systemic concentrations of the antimicrobial coating agents. Colonization of antimicrobial-coated devices was not associated with resistant S. aureus isolates.

Conclusions: These results suggest that silver-coated sewing rings may not prove to be clinically anti-infective. In contrast, antimicrobial-coated sewing rings that produce effective zones of inhibition, particularly those coated with M/R, are likely to be clinically protective.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / administration & dosage*
  • Anti-Bacterial Agents / therapeutic use
  • Anti-Infective Agents, Local / administration & dosage*
  • Anti-Infective Agents, Local / therapeutic use
  • Antibiotic Prophylaxis
  • Bacterial Adhesion / drug effects
  • Chlorhexidine / administration & dosage
  • Chlorhexidine / therapeutic use
  • Coated Materials, Biocompatible*
  • Colony Count, Microbial
  • Disease Models, Animal
  • Drug Combinations
  • Female
  • Heart Valve Prosthesis / adverse effects*
  • Minocycline / administration & dosage
  • Minocycline / therapeutic use
  • Prosthesis Design
  • Prosthesis-Related Infections / prevention & control*
  • Rabbits
  • Rifampin / administration & dosage
  • Rifampin / therapeutic use
  • Silver Sulfadiazine / administration & dosage
  • Silver Sulfadiazine / therapeutic use
  • Staphylococcal Infections / prevention & control


  • Anti-Bacterial Agents
  • Anti-Infective Agents, Local
  • Coated Materials, Biocompatible
  • Drug Combinations
  • Minocycline
  • Chlorhexidine
  • Rifampin
  • Silver Sulfadiazine