Isolation and characterisation of the acetyl-CoA carboxylase gene from Aspergillus nidulans

Curr Genet. 1998 Dec;34(5):379-85. doi: 10.1007/s002940050410.

Abstract

The putative gene encoding acetyl-CoA carboxylase, accA, has been isolated from Aspergillus nidulans. This single-copy gene has an open reading frame (ORF) of 6864 bp and contains two small introns near the 5'-end. A short ORF upstream of the ATG start codon has been identified in this gene by RT-PCR. Based on sequence homology to acetyl-CoA carboxylases from other organisms, putative biotin-, ATP-, HCO3-- and acetyl-CoA- binding sites have been assigned. Northern data and ACC enzyme-activity measurements from A. nidulans suggested that expression of accA was higher in media containing nitrate than ammonia as a sole nitrogen source. Deletion of accA in A. nidulans was unsuccessful. The failure of A. nidulans to grow in the presence of the ACC-specific inhibitor, soraphen A, supplemented with C16-18 fatty acids suggested that ACC is an essential enzyme.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyl-CoA Carboxylase / genetics*
  • Acetyl-CoA Carboxylase / metabolism
  • Amino Acid Sequence
  • Aspergillus nidulans / cytology
  • Aspergillus nidulans / enzymology
  • Aspergillus nidulans / genetics*
  • Base Sequence
  • Cell Division / drug effects
  • Culture Media / pharmacology
  • Culture Media, Conditioned / chemistry
  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics
  • DNA, Fungal / isolation & purification
  • Genes, Fungal / genetics*
  • Molecular Sequence Data

Substances

  • Culture Media
  • Culture Media, Conditioned
  • DNA, Fungal
  • Acetyl-CoA Carboxylase

Associated data

  • GENBANK/Y15996