Abstract
A number of non-natural N-acyl derivatives of glucosamine is incubated with a recombinant beta(1-3)galactosyl-transferase and UDP-galactose. Surprisingly, the enzyme recognizes the non-natural acceptors as substrates and transfers galactose onto the 3-OH group in a beta-mode to give a series of Lewis(c)-(type 1) disaccharides.
MeSH terms
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Carbohydrate Conformation
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Disaccharides / chemical synthesis*
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Disaccharides / chemistry
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Galactose
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Galactosyltransferases / metabolism*
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Glucosamine / analogs & derivatives
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Indicators and Reagents
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Magnetic Resonance Spectroscopy
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Models, Molecular
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Recombinant Proteins / metabolism
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Substrate Specificity
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Uridine Diphosphate Galactose
Substances
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Disaccharides
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Indicators and Reagents
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Recombinant Proteins
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Uridine Diphosphate Galactose
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Galactosyltransferases
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Glucosamine
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Galactose