On the preparative use of recombinant beta(1-3)galactosyl-transferase

Bioorg Med Chem Lett. 1998 Apr 7;8(7):751-4. doi: 10.1016/s0960-894x(98)00093-6.

Abstract

A number of non-natural N-acyl derivatives of glucosamine is incubated with a recombinant beta(1-3)galactosyl-transferase and UDP-galactose. Surprisingly, the enzyme recognizes the non-natural acceptors as substrates and transfers galactose onto the 3-OH group in a beta-mode to give a series of Lewis(c)-(type 1) disaccharides.

MeSH terms

  • Carbohydrate Conformation
  • Disaccharides / chemical synthesis*
  • Disaccharides / chemistry
  • Galactose
  • Galactosyltransferases / metabolism*
  • Glucosamine / analogs & derivatives
  • Indicators and Reagents
  • Magnetic Resonance Spectroscopy
  • Models, Molecular
  • Recombinant Proteins / metabolism
  • Substrate Specificity
  • Uridine Diphosphate Galactose

Substances

  • Disaccharides
  • Indicators and Reagents
  • Recombinant Proteins
  • Uridine Diphosphate Galactose
  • Galactosyltransferases
  • Glucosamine
  • Galactose