Abstract
Two fluorescent substrates for protein tyrosine phosphatase (PTPase) reaction were prepared by conjugation of commercially available O-phosphotyrosine and dansyl chlorides. They were hydrolyzed by CD45 tyrosine phosphatase, and proved to be useful for PTPase assay.
MeSH terms
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Cell Membrane / enzymology
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Dansyl Compounds / chemistry
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Fluorescent Dyes / chemical synthesis*
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Fluorescent Dyes / chemistry
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Humans
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Jurkat Cells
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Leukocyte Common Antigens / chemistry*
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Leukocyte Common Antigens / metabolism*
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Phosphotyrosine / analogs & derivatives*
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Phosphotyrosine / chemical synthesis
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Phosphotyrosine / chemistry
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Sulfonamides / chemical synthesis*
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Sulfonamides / chemistry
Substances
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Dansyl Compounds
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Fluorescent Dyes
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N-(5-(dimethylamino)naphthylsulfonyl)phosphotyrosine
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N-(6-(dimethylamino)naphthylsulfonyl)phosphotyrosine
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Sulfonamides
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Phosphotyrosine
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Leukocyte Common Antigens