Expression of a functional barley sucrose-fructan 6-fructosyltransferase in the methylotrophic yeast Pichia pastoris

FEBS Lett. 1998 Dec 4;440(3):356-60. doi: 10.1016/s0014-5793(98)01487-2.

Abstract

The cDNA encoding sucrose-fructan 6-fructosyltransferase (6-SFT) from barley (Hordeum vulgare) has been expressed in the methylotrophic yeast Pichia pastoris, using a translational fusion into vector pPICZ alphaC, containing the N-terminal signal sequence of Saccharomyces cerevisiae alpha-factor to allow entry into the secretory pathway. Transformed Pichia produced and secreted a functional 6-SFT which had characteristics similar to the barley enzyme, but had a pronounced additional 1-SST activity when incubated with sucrose.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Untranslated Regions
  • DNA, Complementary / analysis
  • Genetic Vectors
  • Glycoside Hydrolases / metabolism
  • Glycosylation
  • Hexosyltransferases / genetics*
  • Hordeum / enzymology*
  • Hordeum / genetics
  • Pichia / genetics*
  • Saccharomyces cerevisiae / genetics
  • Sucrose / metabolism
  • Transfection
  • Transformation, Genetic
  • beta-Fructofuranosidase

Substances

  • 5' Untranslated Regions
  • DNA, Complementary
  • Sucrose
  • Hexosyltransferases
  • sucrose - fructan 6-fructosyltransferase
  • Glycoside Hydrolases
  • beta-Fructofuranosidase