The transcriptional program in the response of human fibroblasts to serum

Science. 1999 Jan 1;283(5398):83-7. doi: 10.1126/science.283.5398.83.


The temporal program of gene expression during a model physiological response of human cells, the response of fibroblasts to serum, was explored with a complementary DNA microarray representing about 8600 different human genes. Genes could be clustered into groups on the basis of their temporal patterns of expression in this program. Many features of the transcriptional program appeared to be related to the physiology of wound repair, suggesting that fibroblasts play a larger and richer role in this complex multicellular response than had previously been appreciated.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blood*
  • Calcium-Calmodulin-Dependent Protein Kinases / genetics
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism
  • Cell Cycle / genetics*
  • Cell Line
  • Cholesterol / biosynthesis
  • Culture Media
  • Culture Media, Serum-Free
  • Expressed Sequence Tags
  • Fibroblasts / cytology
  • Fibroblasts / physiology*
  • Fluorescent Dyes
  • Gene Expression Regulation*
  • Genes, Immediate-Early
  • Humans
  • Oligonucleotide Array Sequence Analysis
  • Polymerase Chain Reaction / methods
  • Software
  • Time Factors
  • Transcription Factors / genetics
  • Transcription, Genetic*
  • Wound Healing / genetics*


  • Culture Media
  • Culture Media, Serum-Free
  • Fluorescent Dyes
  • Transcription Factors
  • Cholesterol
  • Calcium-Calmodulin-Dependent Protein Kinases