Functionalization of a viscosity-sensitive fluorophore for probing of biological systems

Bioorg Med Chem Lett. 1998 Jun 16;8(12):1455-60. doi: 10.1016/s0960-894x(98)00241-8.


Functionalization of a viscosity-sensitive visible wavelength fluorophore 2-(1,1-dicyanopropenyl-2)-6-dimethylaminonaphthalene (DDNP), with the intent to incorporate its favorable optical properties into a probe for structural and functional imaging by fluorescence microscopy, is described. Spiperone, a highly potent ligand for the dopamine D2 receptors, was conjugated via an ethylpiperazine moiety to the fluorophore giving fluorescent probes that can be excited in the UV and Vis range.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • 2-Naphthylamine / analogs & derivatives*
  • 2-Naphthylamine / chemistry
  • Acrylonitrile / analogs & derivatives*
  • Acrylonitrile / chemistry
  • Fluorescence
  • Fluorescent Dyes / chemistry*
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Radioligand Assay
  • Receptors, Dopamine D2 / metabolism
  • Receptors, Serotonin / metabolism
  • Spectrophotometry, Ultraviolet
  • Spiperone / metabolism


  • 2-(1,1-dicyanopropenyl-2-)-6-dimethylaminonaphthaleney
  • Fluorescent Dyes
  • Receptors, Dopamine D2
  • Receptors, Serotonin
  • Spiperone
  • 2-Naphthylamine
  • Acrylonitrile