First synthesis of alternating SATE-phosphotriester/phosphodiester prooligonucleotides on solid support

Bioorg Med Chem Lett. 1998 Oct 20;8(20):2913-8. doi: 10.1016/s0960-894x(98)00518-6.

Abstract

Prooligonucleotides with alternating S-Acyl-ThioEthyl (SATE) phosphotriester and phosphodiester linkages or a gap were synthesized by elimination of cyanoethyl group with non-nucleophilic base DBU. Their half-lives in the presence of Pig Liver Esterases (PLE) and in total cell extract were determined.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Extracts
  • Esterases / metabolism
  • Half-Life
  • Liver / enzymology
  • Liver / metabolism
  • Oligonucleotides / chemical synthesis*
  • Swine
  • Thymidine / chemistry

Substances

  • Cell Extracts
  • Oligonucleotides
  • Esterases
  • Thymidine