Membrane topology of the myelin/oligodendrocyte glycoprotein

Eur J Biochem. 1998 Dec 1;258(2):478-84. doi: 10.1046/j.1432-1327.1998.2580478.x.


Myelin/oligodendrocyte glycoprotein (MOG), a specific component of the mammalian central nervous system, is located on the surface of the oligodendrocyte plasma membrane and the outermost lamellae of mature myelin; it is expressed during the latter steps of myelinogenesis. It has been shown that MOG may play a pathological role in autoimmune demyelinating diseases of the central nervous system, although its physiological function remains unknown. MOG is an integral membrane glycoprotein with an extracellular immunoglobulin-like domain and two hydrophobic segments which were predicted to be membrane-spanning on the basis of hydropathy analysis. As a first step in elucidation of MOG function, we have investigated its membrane topology, combining immunofluorescence studies on cultured oligodendrocytes and MOG-transfected Chinese hamster ovary cells with biochemical analyses, including in vitro translation, membrane insertion and protease-digestion assays. Our results indicate that the C-terminal tail of MOG is located into the cytoplasm, and that only the first hydrophobic region of MOG spans the membrane whereas the second hydrophobic region appears to be semi-embedded in the lipid bilayer, lying partially buried in the membrane with its N-terminal and C-terminal boundaries facing the cytoplasm.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • CHO Cells
  • Carboxypeptidases / metabolism
  • Cathepsin A
  • Cricetinae
  • Endopeptidase K / metabolism
  • Humans
  • Immunohistochemistry
  • Membrane Glycoproteins / chemistry
  • Microsomes / metabolism
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed / genetics
  • Myelin Proteins
  • Myelin-Associated Glycoprotein / chemistry*
  • Myelin-Associated Glycoprotein / genetics
  • Myelin-Oligodendrocyte Glycoprotein
  • Nerve Tissue Proteins / chemistry
  • Oligodendroglia / chemistry
  • Protein Biosynthesis / genetics
  • Transcription, Genetic / genetics
  • Transfection / genetics


  • MOG protein, human
  • Membrane Glycoproteins
  • Myelin Proteins
  • Myelin-Associated Glycoprotein
  • Myelin-Oligodendrocyte Glycoprotein
  • Nerve Tissue Proteins
  • Carboxypeptidases
  • CTSA protein, human
  • Cathepsin A
  • Endopeptidase K