A general method for isolation of high molecular weight DNA from eukaryotes

Nucleic Acids Res. 1976 Sep;3(9):2303-8. doi: 10.1093/nar/3.9.2303.

Abstract

A new method for isolation of high molecular weight DNA from eukaryotes is presented. This procedure allows preparation of DNA from a variety of tissues such as calf thymus or human placenta and from cells which were more difficult to lyse until now (e.g. Crypthecodinium cuhnii, a dinoflagellate). The DNA obtained in such a way has an average molecular weight of about 200 X 10(6) d and contains very few, if any, single strand breaks.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cattle
  • DNA / isolation & purification*
  • Dinoflagellida / analysis
  • Female
  • Humans
  • Liver / analysis
  • Methods
  • Mice
  • Molecular Weight
  • Placenta / analysis
  • Pregnancy
  • Thymus Gland / analysis
  • Ultracentrifugation

Substances

  • DNA