Comparison of the crystal and solution structures of two RNA oligonucleotides

Biophys J. 1999 Jan;76(1 Pt 1):65-75. doi: 10.1016/S0006-3495(99)77178-X.

Abstract

Until recently, there were no examples of RNAs whose structures had been determined by both NMR and x-ray crystallography, and thus there was no experimental basis for assessing the accuracy of RNA solution structures. A comparison of the solution and the crystal structures of two RNAs is presented, which demonstrates that NMR can produce solution structures that resemble crystal structures and thus validates the application to RNA of a methodology developed initially for the determination of protein conformations. Models for RNA solution structures are appreciably affected by the parameters used for their refinement that describe intramolecular interactions. For the RNAs of interest here, the more realistic those parameters, the greater the similarity between solution structures and crystal structures.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Biophysical Phenomena
  • Biophysics
  • Computer Simulation
  • Crystallography, X-Ray
  • Magnetic Resonance Spectroscopy
  • Models, Molecular
  • Nucleic Acid Conformation
  • Oligoribonucleotides / chemistry*
  • Phosphorus / chemistry
  • RNA / chemistry*
  • Solutions
  • Static Electricity
  • Thermodynamics

Substances

  • Oligoribonucleotides
  • Solutions
  • Phosphorus
  • RNA