Identification of Domains Involved in Superantigenicity of Streptococcal Pyrogenic Exotoxin F (SpeF)

Microb Pathog. 1998 Nov;25(5):279-90. doi: 10.1006/mpat.1998.0234.

Abstract

A series of 11 synthetic peptides of 30 amino acids, each with 10 amino acids overlap which spanned the entire sequence of streptococcal pyrogenic exotoxin F (SpeF), were employed in proliferation studies on human peripheral blood mononuclear cells (PBMCs). Regions 41-70, 141-170 and 181-210 were identified as important for SpeF-induced lymphocyte activation. Secondary structure predictions of these peptides showed similarities to regions in other superantigens known to be important for T cell mitogenicity. Furthermore, antisera specific to peptides covering amino acids 1-70 and 181-228 were able to inhibit SpeF-induced mitogenicity by 25% when pre-incubated with SpeF prior to PBMC activation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / chemistry
  • Amino Acid Sequence
  • Animals
  • Antibodies, Bacterial / biosynthesis
  • Bacterial Proteins*
  • Blotting, Western
  • Enzyme-Linked Immunosorbent Assay
  • Exotoxins / chemistry*
  • Exotoxins / immunology
  • Hemocyanins / chemistry
  • Lymphocyte Activation
  • Membrane Proteins*
  • Molecular Sequence Data
  • Peptides / chemical synthesis
  • Protein Structure, Secondary
  • Pyrogens / chemistry*
  • Pyrogens / immunology
  • Rabbits
  • Streptococcal Infections / immunology
  • Streptococcus pyogenes / chemistry
  • Streptococcus pyogenes / immunology*
  • Superantigens / chemistry*
  • Superantigens / immunology

Substances

  • Adjuvants, Immunologic
  • Antibodies, Bacterial
  • Bacterial Proteins
  • Exotoxins
  • Membrane Proteins
  • Peptides
  • Pyrogens
  • SpeA protein, Streptococcus pyogenes
  • Superantigens
  • erythrogenic toxin
  • Hemocyanins
  • keyhole-limpet hemocyanin