cDNA of eight nuclear encoded subunits of NADH:ubiquinone oxidoreductase: human complex I cDNA characterization completed

Biochem Biophys Res Commun. 1998 Dec 18;253(2):415-22. doi: 10.1006/bbrc.1998.9786.

Abstract

NADH:ubiquinone oxidoreductase (complex I) is an extremely complicated multiprotein complex located in the inner mitochondrial membrane. Its main function is the transport of electrons from NADH to ubiquinone, which is accompanied by translocation of protons from the mitochondrial matrix to the intermembrane space. Human complex I appears to consist of 41 subunits of which 34 are encoded by nDNA. Here we report the cDNA sequences of the hitherto uncharacterized 8 nuclear encoded subunits, all located within the hydrophobic protein (HP) fraction of complex I. Now all currently known 41 proteins of human NADH:ubiquinone oxidoreductase have been characterized and reported in literature, which enables more complete mutational analysis studies of isolated complex I-deficient patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cattle
  • Cell Nucleus / enzymology*
  • Cell Nucleus / genetics*
  • DNA, Complementary / isolation & purification*
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Evolution, Molecular
  • Female
  • Humans
  • Male
  • Middle Aged
  • Mitochondria / enzymology
  • Mitochondria / genetics
  • Molecular Sequence Data
  • NAD(P)H Dehydrogenase (Quinone) / chemistry*
  • NAD(P)H Dehydrogenase (Quinone) / genetics*
  • NAD(P)H Dehydrogenase (Quinone) / isolation & purification
  • Neurospora crassa / enzymology
  • Neurospora crassa / genetics

Substances

  • DNA, Complementary
  • NAD(P)H Dehydrogenase (Quinone)

Associated data

  • GENBANK/AF044954
  • GENBANK/AF044955
  • GENBANK/AF044957
  • GENBANK/AF044958
  • GENBANK/AF050637
  • GENBANK/AF050639
  • GENBANK/AF087659
  • GENBANK/AF087660
  • GENBANK/AF087661