Influenza A Virus Lacking the NS1 Gene Replicates in Interferon-Deficient Systems

Virology. 1998 Dec 20;252(2):324-30. doi: 10.1006/viro.1998.9508.

Abstract

The NS1 protein is the only nonstructural protein encoded by influenza A virus. It has been proposed that the NS1 performs several regulatory functions during the viral replication cycle, including the regulation of synthesis, transport, splicing, and translation of mRNAs. Through the use of reverse genetics, a viable transfectant influenza A virus (delNS1) which lacks the NS1 gene has been generated. Our results indicate that the NS1 of influenza A virus is an auxiliary (virulence) factor which plays a crucial role in inhibiting interferon-mediated antiviral responses of the host.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Chick Embryo
  • Chlorocebus aethiops
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / physiology
  • Dogs
  • Gene Deletion*
  • Influenza A virus / genetics*
  • Influenza A virus / physiology
  • Kidney
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Orthomyxoviridae Infections / genetics
  • Orthomyxoviridae Infections / physiopathology*
  • STAT1 Transcription Factor
  • Signal Transduction
  • Trans-Activators / genetics
  • Trans-Activators / physiology
  • Transfection
  • Vero Cells
  • Viral Nonstructural Proteins / genetics*
  • Viral Nonstructural Proteins / metabolism
  • Virus Replication*

Substances

  • DNA-Binding Proteins
  • INS1 protein, influenza virus
  • STAT1 Transcription Factor
  • Stat1 protein, mouse
  • Trans-Activators
  • Viral Nonstructural Proteins