Absence of 60-Hz, 0.1-mT Magnetic Field-Induced Changes in Oncogene Transcription Rates or Levels in CEM-CM3 Cells

Biochim Biophys Acta. 1998 Dec 22;1443(3):334-42. doi: 10.1016/s0167-4781(98)00238-3.

Abstract

Our objective was to assess the reproducibility of the 60-Hz magnetic field-induced, time-dependent transcription changes of c-fos, c-jun and c-myc oncogenes in CEM-CM3 cells reported by Phillips et al. (Biochim. Biophys. Acta, 1132 (1992) 140-144). Cells were exposed to a 60-Hz magnetic field (MF) at 0.1 mT (rms), generated by a pair of Helmholtz coils energized in a reinforcing (MF) mode, or to a null magnetic field when the coils were energized in a bucking (sham) mode. After MF or sham exposure for 15, 30, 60 or 120 min, nuclei and cytoplasmic RNA were extracted. Transcription rates were measured by a nuclear run-on assay, and values were normalized against either their zero-time exposure values, or against those of the c-G3PDH (housekeeping) gene at the same time points. There was no significant difference, at P=0.05, detected between MF and either sham-exposed or control cells at any time point. Transcript levels of the oncogenes were measured by Northern analysis and normalized as above. No significant difference (P=0.05) in transcript levels between MF and either sham-exposed or control cells was detected.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Electromagnetic Fields*
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Oncogenes / genetics*
  • Proto-Oncogene Proteins c-fos / genetics
  • Proto-Oncogene Proteins c-jun / genetics
  • Proto-Oncogene Proteins c-myc / genetics
  • RNA / genetics
  • RNA / metabolism*
  • Transcription, Genetic
  • Tumor Cells, Cultured / cytology
  • Tumor Cells, Cultured / metabolism

Substances

  • Proto-Oncogene Proteins c-fos
  • Proto-Oncogene Proteins c-jun
  • Proto-Oncogene Proteins c-myc
  • RNA